Protein Full-Length Sequencing Service

Description : Protein full-length sequencing is a technique that uses mass spectrometry to fully sequence a protein. Full-length protein sequencing plays an important role in biological and medical research because it can provide comprehensive information about a protein, including its amino acid sequence, modification sites, and chemical modifications. Moreover, full-length protein sequencing can help us understand the function, structure, and interaction of proteins with other molecules.

The basic principle of full-length protein sequencing is to cut the protein into peptides using enzymatic or chemical methods, then sequence these peptides using mass spectrometry, and finally combine these peptide sequence information with bioinformatics techniques to obtain the full-length sequence of the protein. Among them, mass spectrometry is the core technology of full-length protein sequencing, which can provide accurate mass and amino acid sequence information of peptides.

In general, protein sequence analysis service provided by MtoZ Biolabs includes three steps: digestion of proteins into smaller peptide fragments; tandem LC-MS/MS analysis of peptides; MS/MS data analysis. Traditional protein sequencing only uses trypsin for digestion, which renders the final protein sequence coverage of around 60%. To ensure 100% protein sequence coverage, MtoZ Biolabs uses up to 6 kinds of protein enzymes for protein digestions, eliminating any missing peptides.